Recent research suggests that food sensitivity, post-infectious reactivity, brain-gut interactions, alterations in GI tract and fecal microbiota, and upregulation of the GI mucosal immune system are related to a role for the GI microbiota in the pathogenesis of IBS. There is increasing evidence that changes in the gastrointestinal (GI) microbiota are involved in the outbreak of IBS. A meta-analysis has found that there exists a 3- to 11-fold higher risk of IBS after an episode of gastroenteritis. The four main types are categorized by the presence or absence of different symptoms such as constipation predominant (IBS-C), diarrhea predominant (IBS-D), mixed subtype (IBS-M) and unclassified (IBS-U). In addition, IBS is defined as common disturbances of the bowel characterized by abdominal pain or discomfort existing alongside abdominal distension. Irritable bowel syndrome (IBS) is a type of functional gastrointestinal disorder and a major public health problem that occurs in 9–23% of the population across the world. Taken all the results together, the results support that DW probiotics has a potential as a probiotic medication for patient with IBS via regulating TNF-α and IL-6 protein levels and serum IL-1 and IL-6 levels. We have demonstrated that the given probiotics decreased pro-inflammatory cytokine levels in both the control and Pi-IBS induced mice. In addition, the administration of DW (Daewon) probiotics significantly decreased serum levels of IL-1 and IL-6 ( P < 0.05). The reduction of Pi-IBS symptoms through Trichinella infection and the effects of given probiotics were confirmed by a change in the protein levels of cytokines ( P < 0.05). Pi-IBS-induced immune response was confirmed that PAR-2 mRNA level was significantly increased by Trichinella infection ( P < 0.05). To compare molecular, biological and biochemical evidences of given probiotics with normal and positive control groups in mice, we conducted quantitative reverse transcription polymerase chain reaction (RT-qPCR), western blotting, and cytokine analysis. Pathogen-free male C57L/B6 mice and the Trichinella-infected mice were used to measure the score of abdominal withdrawal reflex (AWR).
boulardii) on the behavioral response, targeted gene expression and pro-inflammatory cytokine levels of Pi (Post infectious)-IBS -induced mice. lactis BB12 and Saccharomyces cerevisiae var. The aim of this study was to investigate the effects of probiotic treatments ( Lactobacillus acidophilus LA5, Bifidobacterium animalis subsp.
Lactobacillus and yeast culture are extensively used in probiotic supplements and traditional treatments for irritable bowel syndrome (IBS). Probiotics have been reported to be the active component used in the treatment of many functional gastrointestinal symptoms and syndromes.